Anti-Hu CD45 Purified PTPRC Monoclonal Antibody

Product Name

Anti-Hu CD45 Purified PTPRC Monoclonal Antibody

View all PTPRC Antibodies

SKU/Catalog Number

M00555-3

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Catalog# M00555-3). Tested in Flow Cytometry, IP, WB, IHC-P, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00555-3)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-28

Isotype

Mouse IgG1

Immunogen

Human thymocytes and T lymphocytes. The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00555-3 is reactive to PTPRC in Human

Applications

M00555-3 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

147.486kDa

Background of PTPRC

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used.
Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
Flow cytometry: Recommended dilution: 1 μg/ml.

Validation Images & Assay Conditions

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Figure 3. Flow Cytometry validation of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) purified

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Figure 1. Immunocytochemistry staining of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).

Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28

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Figure 2. Immunohistochemistry validation of PTPRC using Anti-Hu CD45 Purified PTPRC Monoclonal Antibody (M00555-3).

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28)

Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

For more info on PTPRC, check out the PTPRC Infographic

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In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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